Journal: Materials & Design

Article Title: Multifunctional nanofiber-based dressings in coordination with adipose-derived stem cells for accelerated burn wound healing

doi: 10.1016/j.matdes.2025.113929

Figure Lengend Snippet: Fig. 4. (A) Detection of ADSCs proliferation by ADM coating at different times. Quantification of (B) EGF and (C) FGF2 in ADSCs paracrine products. *** P < 0.001 representing a significant difference as compared with ADM (3 min) and TLWDA.

Article Snippet: Human EGF enzyme-linked immunosorbent assay (ELISA) kit and human FGF2 ELISA kit were purchased from BOSTER (Wuhan, China).

Techniques:

Journal: Neuropsychiatric Disease and Treatment

Article Title: Synapse-Related Serum and P300 Biomarkers Predict the Occurrence of Mild Cognitive Impairment in Depression

doi: 10.2147/NDT.S448312

Figure Lengend Snippet: Comparison of baseline serum indices between DWM group and DWOM group. ( A ) The comparison of serum BDNF levels. ( B ) The comparison of serum FGF2 levels. ( C ) The comparison of serum FGF7 levels. ( D ) The comparison of serum FGF22 levels.

Article Snippet: The baseline serum levels of BDNF (Proteintech, USA, Cat No. KE00096), FGF2 (Proteintech, USA, Cat No. KE00129), FGF7 (Abcam, Britain, Cat No. ab314148) and FGF22 (CUSABIO, China, Cat No. CSB-EL008628HU) in patients were measured using ELISA kits according to the manufacturer’s instructions.

Techniques: Comparison

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Morphology of bone marrow progenitor cells 48 h after infection with Ad-BMP2-bFGF-GFP at a multiplicity of infection (MOI) value of 5000. A , Image under light microscope. B , Image under fluorescence microscope showing the expression of green fluorescent protein-positive cells. Bar = 50 μm.

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Infection, Light Microscopy, Fluorescence, Microscopy, Expressing

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Infection efficiency of Ad-BMP2-bFGF in bone marrow progenitor cells. The infection efficiency was calculated by the number of green fluorescent protein-positive cells/total number of nuclei stained with DAPI x100 (%) (n=3). MOI: multiplicity of infection.

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Infection, Staining

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Expression of bone morphogenetic protein 2 (BMP-2) and basic fibroblast growth factor (bFGF) in bone marrow progenitor cells (BMPCs). A , ELISA assay of BMP2 level secreted by BMPCs at different days after infection. B , ELISA assay of bFGF level secreted by BMPCs at different days after infection. Data are reported as means±SD (n=3). *P<0.05 vs control (ANOVA).

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Expressing, Enzyme-linked Immunosorbent Assay, Infection, Control

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Alkaline phosphatase (ALP) activity in bone marrow progenitor cells (BMPCs). A , ALP activity detected in BMPCs at different days after infection. *P<0.05 vs control. # P<0.05 vs Ad-BMP2-infected BMPCs. B , ALP staining of BMPCs 21 days after infection with Ad-BMP2-bFGF. Many brown-black particles appeared in the cytoplasm, and cells were stained blue. Bar = 100 μm.

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Activity Assay, Infection, Control, Staining

Journal: Brazilian Journal of Medical and Biological Research

Article Title: Osteogenesis induced in goat bone marrow progenitor cells by recombinant adenovirus coexpressing bone morphogenetic protein 2 and basic fibroblast growth factor

doi: 10.1590/1414-431X20132929

Figure Lengend Snippet: Kossa staining of bone marrow progenitor cells 21 days after infection with Ad-BMP2-bFGF or Ad-BMP2. Mineralized calcium deposits were stained as dark nodules. Bar = 400 μm.

Article Snippet: The concentrations of BMP2 and bFGF in the supernatants were determined with a human BMP2 ELISA kit (Boster, China) and a human bFGF ELISA kit (Boster, China), according to the manufacturers' instructions.

Techniques: Staining, Infection

Journal: The Journal of Neuroscience

Article Title: Differential Vesicular Targeting and Time Course of Synaptic Secretion of the Mammalian Neurotrophins

doi: 10.1523/JNEUROSCI.1776-05.2005

Figure Lengend Snippet: Intact processing and biological function of GFP-tagged neurotrophins. GFP-tagged NTs were expressed in COS7 cells, and cell lysates and supernatants were analyzed 2 d after transfection. A, Anti-GFP Western blot reveals dominant presence of the NT precursors in the cell lysates (top, arrows, NT-4 precursor with lower MW than the other NTs) and of the correctly processed mature NTs in the COS cell supernatants (bottom, arrow). The horizontal marks at the left indicate the position of GFP. B, NT-induced fiber outgrowth of PC12 cells via overexpressed TrkB and endogenously expressed TrkA receptors, respectively. PC12 cells were cotransfected at 1 DIV with TrkB and GFP plasmids and were incubated for 3 d with supernatants from COS cells overexpressing (for 2 d) GFP-tagged BDNF, NGF, NT-3, NT-4, or wt GFP, respectively, or were incubated with rhBDNF. C, Quantification of NT-induced fiber outgrowth of PC12 cells after 3 d of incubation with COS cell supernatants and recombinant (rec.) neurotrophins. **Significantly different from all NTs with p < 0.01. D, Anti-BDNF Western blot of COS cells expressing wt BDNF and BDNF-GFP, respectively, and of human recombinant BDNF. The blot indicates comparable amounts of wt BDNF and BDNF-GFP in the supernatants used in E. E, PC12 cell fiber outgrowth assay of supernatants and recombinant BDNF analyzed in D. *Significantly different from EGFP with p < 0.01. Data in C and E are from >100 cells per experiment and from more than two independent preparations for each condition. Error bars represent SEs.

Article Snippet: Recombinant human NTs were purchased from Alamone Labs. Monensin was obtained from Sigma.

Techniques: Transfection, Western Blot, Incubation, Recombinant, Expressing

Journal: The Journal of Neuroscience

Article Title: Differential Vesicular Targeting and Time Course of Synaptic Secretion of the Mammalian Neurotrophins

doi: 10.1523/JNEUROSCI.1776-05.2005

Figure Lengend Snippet: Neutralization of pH in secretory granules by monensin before depolarization speeds up the release of BDNF. Hippocampal neurons were transfected with GFP-tagged NTs at 8 DIV and used for secretion 2 d after transfection A, BDNF-GFP-expressing neuron. B, Time series of the marked box in A at higher magnification. C, Analysis of fluorescence intensity of regions marked in B. Note the pronounced increase in fluorescence intensity after application of monensin (4 μm), which neutralizes pH in previously acidic granules. The letters a- e refer to the pictures shown in B. Note the faster time course of BDNF secretion (compare Fig. 11) and the lack of GFP unquenching after K+-induced depolarization. rel., Relative; stimul., stimulation. Error bars represent SEs.

Article Snippet: Recombinant human NTs were purchased from Alamone Labs. Monensin was obtained from Sigma.

Techniques: Neutralization, Transfection, Expressing, Fluorescence

Journal: The Journal of Neuroscience

Article Title: Differential Vesicular Targeting and Time Course of Synaptic Secretion of the Mammalian Neurotrophins

doi: 10.1523/JNEUROSCI.1776-05.2005

Figure Lengend Snippet: Average time course of NT secretion after neutralization of intragranular pH with monensin. A-D, Hippocampal neurons expressing the different GFP-tagged NTs were treated as in Figure 9. Monensin (4 μm)-induced fluorescence increase and acceleration of the time course of release during subsequent depolarization was observed for all NTs (compare Fig. 11). The τ values represent the results of monoexponential fit curves for n ≥ 4 cells for each NT. E, Normalized average depolarization induced (50 mm K+) fluorescence decrease for indicated NTs after preincubation with monensin and for FM4-64 destaining of synaptic vesicles, respectively. The vertical line indicates start of depolarization. Average over n ≥ 4 cells for each condition. F, The boxed area in E at an expanded time scale. Note the similar half decay times (gray circles) for all NTs under these conditions but the still markedly faster speed of FM4-64 release from synaptic vesicles. Stimul., Stimulation. Error bars represent SEs.

Article Snippet: Recombinant human NTs were purchased from Alamone Labs. Monensin was obtained from Sigma.

Techniques: Neutralization, Expressing, Fluorescence